PBIO 8100:
Plant Molecular and Cellular Biology I
|
Fall 2006 |
|
MWF 11:15-12:05 am Rm 1503 |
|
Thurs 11:00-12:15 am Rm 2507 |
| Instructors: | Kelly
Dawe 542-1658/Office - Plant Sciences 4611 Xiaoyu Zhang 542-4822/Office - Plant Sciences 4409 |
| Grading: | Exams
are 75% (25% each) of the course grade. Discussion groups are 25% of the course grade. |
Discussion groups: Primary literature will be assigned and discussed on designated days. ***Each student is expected to understand the methods, interpretation of data, experimental design and controls, and choice of experimental system. You will be asked to voice your views and summarize figures or sections of the papers.
Assigned Readings: The assigned research papers are linked below. Lecture material will be derived primarily from review articles. NOTE: lecture notes are here to give you a glimpse of what will be discussed. I wouldn't print them out just yet because I may change them, and because I'll hand them out during class anyway.
Syllabus for Dawe portion - ONLY ACCURATE TO 8/26 right now
| Week 1: INTRO TO MENDEL | |
| 8/17 |
Lecture: Intro to Mendel (lecture notes) |
| 8/19 |
Paper:Creighton and McClintock (1931). A correlation of cytological and genetical crossing over in Zea mays. Proc. Nat. Acad. Sci. 17, 492-497 |
| 8/20 | Lecture: Chromosomal mutations and Mapping (lecture notes) |
| 8/21 | Lecture: Point mutants (lecture notes) |
| Week 2: MUTATIONS | |
| 8/24 |
Paper: Kaya et al (2000). hosoba toge toge, a syndrome caused by a large chromosomal deletion associated with a T-DNA insertion in Arabidopsis. Plant Cell Physiol. 41, 1055-1066 |
| 8/26 |
Lecture: TDNA (lecture notes) |
| 8/27 | Paper: Buck et al (2009. The T-DNA Integreation pattern in Arabidopsis transformants is highly determined by the transformed target cell. Plant J. in press, but available as epub ahead of print. |
| 8/28 | Lecture: Unstable mutations and other forms of tagging. |
| Week 3: T-DNA and TRANPSOSON-INDUCED MUTATIONS & CELL AUTONOMY | |
| 8/31 | Paper: Settles et al. (2007). Sequence-indexed mutations in maize using the UniformMu transposon-tagging population. BMC genomics 8:116 |
| 9/2 |
Paper: Townsend et al. (2009). High-frequency modification of plant genes using engineered zinc-finger nucleases. Nature 459: 442-445. . |
| 9/3 | Lecture: Cytoplasmic mutations and cell autonomy (lecture) |
| 9/4 | Paper: Shikanai et al (2001). The chloroplast clpP gene, encoding a proteolytic subunit of ATP-dependent protease, is indispensable for chloroplast development in tobacco. Plant Cell Physiol. 42, 264-73. |
| Week 4: DOMINANT MUTATIONS | |
| 9/7 | Labor Day no class |
| 9/9 | Lecture: Dominant mutations (lecture) |
| 9/10 |
Paper: Gosti et al (1999). ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11, 1897-1909 |
| 9/11 |
Paper: Hanumappa et al (2007). Modulation of flower colour by rationally designed dominant negative chalcone synthase. J. Exp. Botany 58: 2471-2478 |
| Week 5: EPISTASIS | |
| 9/14 | Lecture: Epistasis (lecture) |
| 9/16 |
Paper: Luo and Oppenheimer (1999) Genetic control of trichome branch number in Arabidopsis: the roles of the FURCA loci. Development 126: 5547-5557 |
| 9/17 |
Lecture: Suppressors (lecture) |
| 9/18 |
Paper: Steber et al. (1998) Isolation of the GA-response mutant sly1 as a suppressor of ABI1-1 in Arabidopsis thaliana. Genetics 149: 509-521 |
| Week 6: Reverse Genetics | |
| 9/21 |
review :EXAMPLE QUESTIONS |
| 9/23 |
Exam I |
| 9/24 |
Lecture: Reverse Genetics |
| 9/25 | Lecture: Activation tagging |
| Week 7: Activation tagging, Genomes | |
| 9/28 |
Paper: Chuang and Meyerowitz (2000). Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Plant Cell 97: 4985-4990 |
| 9/30 |
Paper: (Yu et al. 2008) Activated Expression of an Arabidopsis HD-START Protein Confers Drought Tolerance with Improved Root System and Reduced Stomatal Density. Plant Cell 20:1134-1151 |
| 10/1 |
Lecture: Polyploidy |
| 10/2 |
Lecture: Centromeres and positional cloning |
| Week 8: CENTROMERES and TELOMERES | |
| 10/5 | Paper:Han et al. (2009). Centromere repositioning in cucurbit species: Implication of the genomic impact from centromere activation and inactivation. PNAS 106: 14937–14941 |
| 10/7 |
Paper, last day for Dawe: Bortiri et al (2006). ramosa2 Encodes a LATERAL ORGAN BOUNDARY Domain Protein That Determines the Fate of Stem Cells in Branch Meristems of Maize. Plant Cell 18: 574-585. |
| 10/8 |
Lecture: Dr. Zhang starts teaching. He will talk about the structure of plant genes. |
| 10/9 | -------- |
| Week 9: | |
| 10/12 | ---------- |