Transposon display (TD) is a modification of the AFLP
technique that uses a primer anchored in a transposon to simultaneously
detect up to several hundred markers in the genome. TD involves the amplification
of sequences flanking the transposon by ligation-mediated PCR, so the resulting
fragments are locus-specific and can be analyzed by polyacrylamide gel
electrophoresis. TD has been used to study the behavior and stability of
transposable elements, including retrotransposons, in various organisms
(Flavell et al., 1998; Van den Broeck et al., 1998; Waugh et al., 1997;
Purugganan and Wessler 1995). Thus, both DNA and RNA transposons can be
utilized in TD to generate molecular markers. Current marker technologies
have their own advantages and disadvantages. RFLPs are reproducible but
difficult to perform and/or yield a low density of markers. RAPDs are straightforward
but lack reproducibility. AFLPs yield a high number of markers but they
tend to cluster in heterochromatin regions. SSRs are likewise straightforward
but require prior sequence information. TD has several potential advantages
over these technologies when coupled with a particular class of transposable
elements known as MITEs.

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