TEs in Rice

University of Georgia
Dept of Plant Biology
Athens, GA 30602
Phone:(706)542-1857
Fax:(706)542-1805 

 

 
COLLABORATORS
Dr. Susan Wessler 
University of Georgia
Dr. Susan McCouch
Cornell University
Dr. Sean Eddy
WashingtonUniversity
 

PUBLICATIONS
 

RESEARCH OPPORTUNITIES
(Graduate, undergraduate & post-doc)
 

PHOTOS
 

EMPLOYMENT
 

PROJECT FUND
The National Science Foundation
 

PROJECT LINKS
TIGR Rice Genome Project
Monsanto Rice Genome Project
Genome Database of Chinese Super Hybrid Rice
The Plant Genome Database
National Center for Biotechnology Information
The Gramene Database
The Rice Genome Program (RGP) in Japan
US Rice Genome Sequencing Project 
Clemson Rice Project
Cold Spring Harbor Lab Rice Project


 

Transposable Elements in Rice: A Whole Genome Approach

A large fraction of the DNA sequence output from plant genome projects will be derived from transposable elements (TEs).  It is estimated that  25% of the small genome of rice (Oryza sativa cv. Nipponbare, 438 Mb),  is derived from TEs.   What distinguishes TEs from the genic complement of an organism, among other things, is an ability to transpose and amplify.  Thus, TEs shake up an otherwise conservative genome and, in this way, both threaten and enhance genomic potential.



PROJECT GOAL
Here we will take a  functional genomics approach to rice transposable elements (TEs) with three objectives:

(1) identification and characterization of most of the TE families in the rice genome 
(Eddy, McDonald, Wessler)

(2) determination of TE activity and quantification of TE-mediated diversity in Oryza sativa cultivars and in Oryza subspecies and related species 
(Wessler, McCouch)

(3) assessment of the contribution of TE-mediated diversity to phenotypic variation 
(McCouch, Wessler)



EXPERIMENTAL OBJECTIVES:
  • computationally identify rice TEs and phylogenetically characterize TE families.  Test two new repeat element identification algorithms
  • identify active TE families by assaying a panel of wide-species crosses and a variety of stress environments
  • determine the chromosomal distribution of ~1000 TE insertions sites that are polymorphic in a Nipponbare/IR64 mapping population and develop a subset into anchor markers
  • use the mapping populations and anchor markers as the basis for correlating TE-based genetic variation with phenotypic variation for selected plant traits




Transposable elements probably account for the huge difference in size among the agronomically important members of the grass clade including rice, sorghum, maize and wheat.  The proposed consortium will serve as a model for how TEs should be studied in these more complex genomes.
 
 The Wessler Lab Homepage

dawn@plantbio.uga.edu
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Last modified: 06/03